The Genotyping Kit Venous Thrombosis allows the determination of the R/Q 506 mutation in the factor V gene Factor V Leiden, the 20210 G/A mutation in the prothrombin Factor II gene and A/V 223 677 C/T mutation in the 5,10-methylene-tetrahydrofolate reductase gene MTHFR. The assay consists of three steps: 1. DNA extraction2. DNA amplification by PCR 3. analysis of PCR products by gel- electrophoresis. Step 2 is based on a multiplex PCR reaction, which combines multiple primer combinations in the same reaction tube. In this kit 6 specific primer combinations are used to amplify mutant as well as wild type normal variant alleles of three different genes. Thus, homozygous and heterozygous carriers of the mutations can be identified by one PCR test. The Genotyping Kit contains DNA diluent sterile 2x3 mL, mastermix for 2x11 PCR reactions2x360 L, size marker 1x20 L, SybrGreen I 5L sample, 2 ready to use Spreadex EL 400 Mini gels, 1 gel frame and the Electrophoresis buffer 40 x concentrated. SybrGreen is a trademark of Molecular Probes, Inc.,USA Spreadex is a trademark of Elchrom Scientific AG
Storage temperature: +2-8C Transport temperature: + 2...8C Supplier: Technoclone GmbH
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