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Stem cells are cell types that are know for being hard to
transfect. Still the manipulation of the stem cell genome is an
essential means in stem cell focused research. There are different
reagents available depending on what type of construct that is used in
the transfection process.
siRNA
Lullaby Stem has
been optimized for high transfection rates of stem cells using siRNA or
miRNA. This reagent is based on the TEE-technology which protects small
RNA from extracellular degredation at the same time enabelling
efficient passage over the cellular plasma membrane.
mRNA
mRNARmesFect Stem has specifically been designed for transfection of stem cells and a large variaty of primary cells. Transfection
of mRNA instead of plasmid DNA has many advantages similar to what
siRNA transfection can offer in knock-down experiments. In the mRNA
based knock-in approach there is no need for nuclear uptake, protein
expression from time of transfection is shorter and the expression is
not dependent on promoters.
DNA
Dreamfect Stem
has a unique design that allow large amount of DNA material to enter
different cell types including MSC (Mesenchymal Stem Cells), AdSC
(Adipose derived Stem Cells), AFSC (Amniotic Fluid Stem Cells), and KG1a
(leukemic stem-like cells) as well as embryonic stem cells.