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Our specially formulated 3D transfection reagents offer multiple benefits when seeding cells on scaffolds our gels. Instead of transfecting first before you expand cells for seeding on scaffolds or hydrogels, seeding and transfection can be done simultaneously by applying our 3D culture reagent which binds to scaffold material allowing transfection to take place while the cells migrate through the construct.
Two different reagent formulations are available depending whether hydrogels or scaffolds are used. The reagents form a complex with the nucleic acids that are used, and the complexes adheres to the scaffold and gel material when added
3D-Fect reagents are used for scaffolds and 3D-FectIN are used when seeding on hydrogels.